HK-1-CAS9 cell line

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HK-1-CAS9 Order

HK-1-CAS9 cell line
Catalog#
YC-C095-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Datasheet
HK-1-CAS9 cell line
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Detailed Product Information

Product category
Cas9 Stable Cell Lines
cell name
HK-1-CAS9
Cell Morphology
Epithelial-like,?adherent
Passage ratio
1:2~1:4
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10% FBS
Description
Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the HK-1 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a HK-1-CAS9 cell line that can maintain long-term, stable and high expression.
Product Validation Data（RT-QPCR）
Sample Name Target Name Cт Mean ΔCт
HK-1-CAS9 Cas9 24.63736534 -1.13356972
HK-1-CAS9 β-actin 25.77093506
HK-1 Cas9 33.87138367 8.65867806
HK-1 β-actin 25.21270561
Cutting Efficiency Validation
HK-1-CAS9 cell line Product Validation Data（RT-QPCR）
Note: The above figure shows the sequencing peaks of the HK-1-CAS9 stable cell pool which is electroporated by NONO gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:HK-1		 STR Info (Cell bank)
Cell Line:NPC/HK1
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 111311.013.0
D13S317 111111.011.0
D7S820 8118.011.0
D16S539 9119.011.0
VWA 181918.019.0
TH01 797.09.0
AMEL XX
TPOX 8118.011.0
CSF1PO 111211.012.0
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
    01
  • High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
    02
  • High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
    03
HK-1-CAS9 cell line
HK-1-CAS9
Catalog#
YC-C095-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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